Composite

Part:BBa_K1022124:Design

Designed by: Maithili Krishnan   Group: iGEM13_TU-Delft   (2013-09-20)

pT7: RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lysis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 46
    Illegal NheI site found at 2361
    Illegal NheI site found at 4456
    Illegal NheI site found at 4479
    Illegal NotI site found at 3057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 170
    Illegal BamHI site found at 3026
    Illegal XhoI site found at 3035
    Illegal XhoI site found at 3066
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 355
    Illegal AgeI site found at 4061
    Illegal AgeI site found at 4131
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4712


Design Notes

Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft]

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Source

Clonned

References